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KMID : 0380020220370020064
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Korean Journal of Biotechnology and Bioengineering
2022 Volume.37 No. 2 p.64 ~ p.70
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Evaluation of Bioactivity of Castanopsis cuspidata var. sieboldii Leaves Extract and Isolation of Polyphenolic Compounds
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Kim Na-In
Choi Moon-Hee
Yang Seung-Hwa
Oh Deuk-Sil
Shin Hyun-Jae
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Abstract
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Castanopsis cuspidata var. sieboldii (CCS) is a warm-tempered tree that lives on the korean peninsula and has been traditionally used in medicine. Bioactive ingredients from aerial parts of CCS have been identified by several researchers along with anti-inflammatory, antioxidant and whitening effects. However, isolation study of compounds centered on antioxidant activity in CCS leaves (CSL) has not been investigated. In this study, CSL were extracted using 70% ethanol and used as a crude extract. CSL was divided into five fractions using n-hexane, chloroform, ethyl acetate, n-butanol and water. Also, the antioxidant activity of subfraction was determined with DPPH free radical scavenging activity assay and ABTS cation radical scavenging activity assay. The antioxidant contents was confirmed by total polyphenol contents (TPC) and total flavonoid contents (TFC). Through the HPLC qualitative analysis, it was confirmed that CSL and CSL-EA contained various polyphenols. Based on these results, we suggest that extracts, fractions and compounds isolated from CSL provide promising antioxidant abilities and natural cosmetic materials.
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KEYWORD
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Castanopsis cuspidata var. sieboldii, polyphenol, flavonoid, antioxidant activity, HPLC analysis, natural cosmetic materials
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